Device

Part:BBa_K1903010:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


Plac-RBS + RFP-IntN DnaB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This device is based on the RFP-Intein N DnaB BBa_K1903009 linked to a Lacl regulated promoter R0010 assembled with the RBS Elowitz BBa_B0034.


We assembled this device and verified it in an agarose gel electrophoresis:


We were able to express the RFP-Intein N with a size of 27KDa:


Source

References